agrisera品牌PEPC磷酸烯醇式丙酮酸羧化酶
background Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) serves as an important control element in the regulation of photosynthetic carbon metabolism in C4 and CAM plants. This is the first enzyme of the pathway, and PEPC enzymes are encoded by a small multigenic family. Several isoforms of PEPC have been characterised in maize, sorghum and sugarcane. These isoforms are involved in several functions such as the initial fixation of atmospheric CO2 (= C4 PEPC) and anaplerotic functions associated with nitrogen assimilation or amino acid biosynthesis (Lepiniec et al. 1994).
antibody format
does not apply
quantity
100 µl, lyophilized, for reconstitution add 90 µl of milliQ water.
Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications western blot (WB)
related products | AS07 241 | anti-PEPCK | PEP carboxy kinase
additional information The PEPC protein standard can be used in a combination with Agrisera global PEPC antibiody to quantitate PEPC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence.
Quantitative western blot: detailed method description, video tutorial
APPLICATION INFORMATION
recommended dilution Standard curve: 3 loads are recommended (0.5, 2 and 4μl).
For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range.
Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.
expected | apparent MW 110 | 105 kDa
additional information Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.
This standard is ready-to-load and does not require any additions or heating.
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